1090. Experimental end-to-side arterial bypass in rat: comparison interrupted and running suture technique.

Authors: Vladimir Priban, MD, PhD; Vladimir Priban, MD, PhD; Jiri Dostal; Pavel Klein, MD, PhD (Plzen, Czech Republic)

Introduction: The choice of either running or interrupted suture technique is important in bypass surgery. The shorter is time of occlusion the lower risk of ischemia results. Blood loss from anastomosis is also important. Authors compared both techniques in experimental arterial model in rat. Methods: Arterial end-to-side bypass was performed in rats using left common carotid artery as donor and right common carotid artery as an recipient. Surgery was performed under general anesthesia and pulse rate plus oximetry were recorded. Flow was measured quantitatively using experimental Charbel probe in left intact carotid artery before bypass procedure. Finally was recorded flow through anastomosis. Time of surgery and blood loss through bypass were recorded as well. After measurement animals were sacrified. Ten Long-Evans rats were operated using interrupted technique and 10 using running suture technique. Results: Interrupted suture technique: Average i nitial flow through intact left carotid artery was 5,0 ml/min. Average bypass flow was 3,83 ml/min that means 76.6% of initial flow. Time of surgery was 53 minutes and blood loss in anastomosis was 2,0 ml. Interrupted suture technique: Average initial flow through intact left carotid artery was 3.75 ml/min. Average bypass flow was 3.75 ml/min that means 100% of initial flow. Time of surgery was 31 minutes which much shorter than in interrupted suture technique. Average blood loss was 2,4 ml- Conclusion: Running suture technique in experimental end-to-side bypass takes shorter time than interrupted suture technique and the bypass flow is higher in acute phase. Average blood los is relatively higher.